Recent advances in the biotoxicity of silica nanoparticles / 医学研究生学报

Silica Nanoparticles (SiNPs) is widely used in many fields including antibacterial agent, molecular probe and drug delivery forits special physicochemical properties. Recently, the biosecurity of nano-materials has become a hot spot of research in toxicology due to special structure and functional activeness of nano-materials. This paper briefly summarized recent researchreports at home and abroadto review the toxicity of SiNPs on cells and animals, the factors for influencing the toxicity of SiNPs and the mechanisms underlying SiNPs biotoxicity, aiming to provide references for the development, application and biosecurity of SiNPs.

Study on effects of different habitat processing methods of Salviae Miltiorrhizae Radix et Rhizoma in rats with acute myocardial ischemia / 中国中药杂志

To investigate the effects of different habitat processing methods of Salviae Miltiorrhizae Radix et Rhizoma on acute myocardial ischemia induced by pituitrin in rats. In this experiment, the tail vein injection of pituitrin was used to induce acute myocardial ischemia in rats. Electrocardiograph(ECG) heart rate and ΔST changes were recorded, and the levels of creatine kinase isoenzyme(CK-MB), lactate dehydrogenase(LDH), superoxide dismutase(SOD) and malondialdehyde(MDA) in serum of rats were detected to comprehensively evaluate the effects of six processing methods of Salviae Miltiorrhizae Radix et Rhizoma on serum biochemical indexes of rats with acute myocardial injury. The ECG results showed that the Salviae Miltiorrhizae Radix et Rhizoma dried in a drying oven had a good effect on the improvement of heart rate and ΔST of electrocardiogram after ischemia, and all the other groups had some protective effects to different degrees. The results of biochemical indexes in serum of each group after ischemia showed that the activity of CK-MB decreased most significantly in Salviae Miltiorrhizae Radix et Rhizoma high-dose group with drying in a drying oven after sweating and losing weight in a drying oven, high-dose group with drying in the shade and low-dose group with drying in the shade. The activity of LDH decreased most significantly in Salviae Miltiorrhizae Radix et Rhizoma high-dose group with drying in the shade and low-dose group of drying in the shade. The activity of SOD increased most significantly in Salviae Miltiorrhizae Radix et Rhizoma low-dose group with drying in sun, low-dose group with drying in sun after sweating and losing weight in sun, and low-dose group with drying in a drying oven. The activity of MDA decreased most significantly in Salviae Miltiorrhizae Radix et Rhizoma low-dose group with drying in sun. The comprehensive scoring results showed that the highest score was obtained in Salviae Miltiorrhizae Radix et Rhizoma high-dose group with drying in the shade while the scores of other treatment groups were higher than that of the model group. It could be seen that the Salviae Miltiorrhizae Radix et Rhizoma dried in a drying oven had a good improvement effect on electrocardiograph indexes after acute myocardial injury, the Salviae Miltiorrhizae Radix et Rhizoma dried in the shade had a good improvement effect on serum myocardial enzymes after acute myocardial injury, and the other processing methods had a certain protective effect on myocardial injury. The six processing methods evaluated by pharmacodynamics showed that the Salviae Miltiorrhizae Radix et Rhizoma dried in the shade and dried in a drying oven had good efficacy.

Effect of moxifloxacin on pro-inflammatory response in lipopolysaccharide-stimulated mice peritoneal macrophages / 基础医学与临床

Objective To investigate the effect of moxifloxacin(MXF)on inflammatory reaction of mice abdominal peritoneal macrophage induced by LPS.Methods The level of TLR4、SPHK1 and NF-κB mRNA was determined by realtime-PCR;The relative levels of TLR4、SPHK1 and NF-κB were determined by Western blot.TNF-αand IL-1 se-creted by macrophages were detected by ELISA.Results At low and medium concentration(8,16 mg/L)of the MXF have inhibitory effect on the increasing of TLR 4,SPHK1,NF-κB expression level and cell supernatant of TNF-αand IL-1 in LPS stimulation of mice peritoneal macrophages.High concentration(64 mg/L)of the MXF promoted the role of inflammation.Conclusions The MXF of low and medium concentrations can inhibit the inflammatory response in LPS stimulation of mice peritoneal macrophages,and high concentration of the MXF showed contrary effects.

With genechip technique to investigate HLA-DQB1 gene polymorphisms in south and north regions of China / 中华医学遗传学杂志

<p><b>OBJECTIVE</b>To research and compare HLA-DQB1 gene frequency(GF) and polymorphism distribution between south and north population of Chinese in China.</p><p><b>METHODS</b>Combining PCR-sequence specific primers(SSP) and sequence specific oligonucleotide probe(SSOP) techniques and DNA Microarray Kit for HLA-DQB1 Low Res Genotyping from Shenzhen Yi-Shengtang Biological LTD. Co. was used to type HLA-DQB1 gene polymorphisms of 700 individuals living in south China and 320 individuals in north China.</p><p><b>RESULTS</b>We inspected 10 alleles of HLA-DQB1 and got a series of comprehensive and accurate statistic data.</p><p><b>CONCLUSION</b>It is tested that HLA-DQB1*02, 05, 0601, 0602, 0603 gene frequencies are different obviously(P<0.05) between south and north Chinese. And those data will be useful to kinds of research associated with disease relevant and anthropology research.</p>

Evaluation on reducing effect of eye drop on herpes simplex virus-I in vitro / 中华实验和临床病毒学杂志

<p><b>OBJECTIVE</b>To observe the effect of herbal eye drop Reduqing (clearing toxic heat) on herpes simplex virus (HSV) on the cytopathic effect in cell culture in vitro.</p><p><b>METHODS</b>The maximal non-toxic Reduqing eye drop concentration added to cell culture infected with 100 and 10TCID50 of herpes simplex virus? (HSV-1) to observe the inhibitory effect of the medicine on HSV-I induced cytopathic effect.</p><p><b>RESULTS</b>The Reduqing eye drop at maximal non-toxic concentration could obviously inhibit the cytopatic effect induced by 100TCID50 and 10TCID50 HSV-I. No statistically significant difference was found when compared with 0.1% acyclovir eye drop (P>0.05), on cytopathic effect occurred with boty eye drops. However, there was significant difference when compared with control group(P<0.05). The inhibitory effect of concentrations of Reduqing at 500,000 and 63,000 microg/L was stronger than that of 2,000,000 and 1,000,000 microg/L (P 0.01). The maximal tolerable concentration of Reduqing eye drop by the cell was 2,000,000 microg/L (1:80 dilution), which was higher than that of 5,000 microg/L(1:400 dilution) acyclovir eye drop.</p><p><b>CONCLUSIONS</b>These results suggest that Reduqing eye drop could markedly inhibit the cytopathic effects caused by HSV-I. The Reduqing eye drop showed remarkably low toxic effect to the cells as compared to acyclovir eye drop.</p>